E. coli Lipopolysaccharides로 유도된 사람 호중구에서 CD14, Toll-Like receptors, cytoskeletal inhibitors 그리고 NF-κB inhibitor가 MMP-8 분비에 미치는 영향
Effect of CD14, Toll-Like receptors, cytoskeletal inhibitors and NF-κB inhibitor on MMP-8 release from human neutrophils induced by E. coli Lipopolysaccharides
소속 상세정보
양승민
김태일/설양조/이용무/구영/정종평/한수부/류인철
KMID : 0363020050350020427
Abstract
Objective: MMP-8 is a neutrophil enzyme and its level increases in some inflammatory
diseases, including periodontal disease. We knew that the lipopolysaccharide of E.coli(E-LPS)
induced MMP-8 release from human neutrophils. E-LPS is known to induce the production
and release of inflammatory cytokines through CD14, Toll-like receptor(TLR). In the present
study, we investigated whether MMP-8 release by E-LPS is induced via CD14-TLR
pathway and the cellular mechanism of MMP-8 release in human neutrophils.
Material and methods: Human neutrophils were isolated from the peripheral blood of
healthy donors and pre-incubated in medium containing antibodies against CD14, anti-TLR2
and anti-TLR4 or several inhibitors of microtubules and microfilaments and then incubated
with E-LPS. The cells were treated TPCK and E-LPS simultaneously. The MMP-8amount
in the culture medium was determined using ELISA.
Results: E-LPS increased MMP-8release from neutrophils and its induction was inhibited
by anti- CD14 and anti-TLR4 but not by anti-TLR2 antibodies. The inhibitors of microtubule and microfilament polymerization significantly decreased E-LPS-induced MMP8release. TPCK inhibited E-LPS-induced MMP-8 release.
Conclusion: These results suggest that MMP-8 release is induced by E-LPS via the
CD14-TLR4 signal pathway in human neutrophils and may be depedent on microtubule and
microfilament systems and NF-κB pathway.
키워드
Human Neutrophil;CD14;TLRs;Cytoskeletal Inhibitors;NF-B inhibitor;MMP-8
원문 및 링크아웃 정보
등재저널 정보